This changelog only lists notable changes for the end user. For all changes, please see the commit history.
- Pixel-wise autofluorescence correction.
- Show corrected donor and acceptor images when creating a FRET image.
- Save FRET images from the main window.
- Copy region of interests (ROIs) in the spectral correction factor calculation windows.
- Background subtraction in the spectral correction factor calculation windows.
- Reset thresholding steps in the spectral correction factor calculation windows.
- Save and load parameters as a CSV file (experimental).
- Negative pixel values are now included when calculating spectral correction factors.
- Gaussian blur sigma (radius) values are now
0by default. - Background subtraction and Gaussian blurring steps have been reordered.
- Spectrum LUT is no longer applied automatically.
- RiFRET now uses Maven. The result is a single distributable JAR.
- Check for updates has been removed in favor of the ImageJ updater.
- Help now points to https://imagej.net/plugins/rifret.
| Old | New |
|---|---|
GaussianBlur.blur() |
GaussianBlur.blurGaussian() |
ResultsTable.addLabel() |
ResultsTable.setValue() |
Java.util.Date |
Java.time |
- Added: Scroll bars to accommodate new features.
- Changed: Improved hard-coded window sizes, particularly on macOS.
- Changed: Text fields for instrument background subtraction are now larger.
- Changed: Use native looking file chooser for I/O.
- Changed: Revised menu.
- Changed: Renamed Use LSM to Use stack.
- Changed: Alpha factor calculation window UI.
- Changed: Semi-automatic has been revised significantly --> batch processing.
- Changed: Various tweaks.
- Fixed: Various typos.
- Log image names when setting channels.
- Log sigma values when applying Gaussian blur.
- Log saving the log!
- Fixed: Button color now changes to green once clicked on macOS.